Forum

Tal 137 subjects enrolled 9 were excluded. All the 128 subjects were male Majority of the subjects was in the age group 21-30 yrs (n = 86)-. Majority were from Nepal 76 (58.1 ). 30 (20.3 ) patients gave history of previous episodes of seizure, however only 8 of them were on regular antiepileptic medication. 107 patients presented to A E with seizures (83.5 ). Among which GTCS was the most common form 85 . Use of tobacco was seen in 20 subjects (13.6 ) and Alchohol in 10 (6.8 ). 9 subjects reported to use both. Radiological examination, CT scan revealedCritical Care 2016, Volume 20 SupplPage 52 ofsolitary lesion in 84 (65.5 ) subjects and majority were calcified 39.8 . Perilesional edema was noted in 100 subjects. 114 patients were admitted to the hospital out of which 5 required ICU care. Anti-epileptic medication was initiated in all patients except 10 patients. Reason could not be identified. Except PRIMA-1 for 13 patients all other patients received short course of oral steroids. Conclusions: 1) Majority of the patients are young males from Asian countries. 2) GTCS is the most common mode of presentation. 3) Solitary lesion is the most common radiological findings. The increased ease of international travel and increasing number of immigration from developing countries have led to widespread recognition of NCC developed countries.Reference 1) Growing frequency of Neurocysticercosis in Spain. Esquivel A. Neurologia 2005,20(3):116-P114 Filtering of red blood cells reduces the inflammatory response of pulmonary cells in an in vitro model of mechanical ventilation M. R. Witrz, J. F. Buchner-Doeven, A. M. Tuip-de Boer, J. C. Goslings, N. P. Juffermans Academic Medical Centre, Amsterdam, Netherlands Critical Care 2016, 20(Suppl 2):P114 Introductions: Red blood cell (RBC) transfusion is associated with acute lung injury, but the causative factor is not known. Possibly, mechanical ventilation and red blood cell (RBC) transfusion are synergistic in the induction of lung injury. We hypothesize that filtering of RBCs or depletion of RBCs from microvesicles decreases the pulmonary inflammatory reaction during mechanical ventilation. The objective of this study is to investigate the effects of filtering of RBCs or depletion of RBCs from microvesicles on the production of IL-8 in an in vitro model of mechanical ventilation. Methods: Alveolar epithelial type II A549 cells were cultured on an elastic membrane and incubated with cells or supernatant from stored (35-42 days) RBC products. RBCs were optimized by filtering of the PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/8627573 PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/22316373 supernatant using a 0.2micron filter, or by depletion of the supernatant from microvesicles using high-speed centrifugation (N = 4 per group). Cyclic stretch of 25 was applied to the cells for 24 hours using a cell-stretcher at 37 #730;C in the presence of 5 CO2. IL-8 production was measured using ELISA. Controls are untreated products and non-stretched cells. Results: Stretched cells showed higher levels of IL-8 compared to non-stretched cells (198.2 ?11.7 vs 62.0 ?1.9 pg/ml, p < 0.05). Incubation with untreated RBC products further spiked IL-8 production compared to cells that were only stretched (892.8 ?235.8 vs 198.2 ?11.7 pg/ml, p = 0.027). Both filtering of supernatant and depletion of microvesicles resulted in reduction in levels of IL-8, compared to samples to which untreated RBCs were added (respectively 306.6 ?73.1 vs 340.2 ?24.4 vs 892.8 ?235.8 pg/ml, p = 0.02). Conclusions: Stretching of alveolar cells and RBC.